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葉綠素?zé)晒庀到y(tǒng)成像儀——PAM-WATCH
日期:2018-12-27 11:21:00

功能強(qiáng)大、操作簡(jiǎn)便、文獻(xiàn)眾多的葉綠素?zé)晒獬上裣到y(tǒng)

突變株快速篩選的強(qiáng)大工具


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?  標(biāo)準(zhǔn)藍(lán)光版,450 nm,測(cè)葉片和真核藻類

?  紅光版,620 nm,測(cè)藍(lán)藻和真核藻類

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主要功能

?  成像功能:對(duì)Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等參數(shù)進(jìn)行成像分析。測(cè)定調(diào)節(jié)性能量耗散Y(NPQ),反映植物光保護(hù)能力,測(cè)定非調(diào)節(jié)性能量耗散Y(NO),反映植物光損傷程度。

?  程序測(cè)量功能:可程序測(cè)量熒光誘導(dǎo)曲線、快速光曲線和暗弛豫,也可手動(dòng)測(cè)量;在測(cè)量過(guò)程中能自動(dòng)分析所有熒光參數(shù)的變化趨勢(shì)

?  AOI功能:可在測(cè)量前或測(cè)量后任意選擇感興趣的區(qū)域(AOI),程序?qū)⒆詣?dòng)對(duì)選擇的AOI的數(shù)據(jù)進(jìn)行變化趨勢(shì)分析,并在報(bào)告文件中顯示相關(guān)AOI的數(shù)據(jù)。所有報(bào)告文件中顯示的數(shù)據(jù)都可導(dǎo)出到EXCEL文件中。

?  成像異質(zhì)性分析功能:對(duì)任意參數(shù)任意時(shí)間的成像,可在圖像上任意選取兩點(diǎn),軟件自動(dòng)對(duì)兩點(diǎn)間的數(shù)據(jù)進(jìn)行橫向異質(zhì)性分析,并可導(dǎo)出到EXCEL文件中。

?  成像數(shù)據(jù)范圍分析功能:對(duì)任意參數(shù)任意時(shí)間的成像,可分析任意兩個(gè)熒光數(shù)值之間有多少個(gè)像素點(diǎn),多少面積(cm2)。

?  突變株篩選功能:可跟據(jù)成像結(jié)果快速篩選光合、產(chǎn)氫/油、抗逆(抗鹽、抗旱、抗病等)等突變株。

?  微藻毒理研究功能:可同時(shí)測(cè)量96個(gè)微藻樣品(對(duì)照和處理組)的光合活性,軟件自動(dòng)給出處理組樣品相對(duì)于對(duì)照組的光合抑制百分比。

?  吸光系數(shù)測(cè)量功能:快速測(cè)量葉片的吸光系數(shù)。吸光系數(shù)測(cè)量光源: 16個(gè)紅光(650 nm)和16個(gè)近紅外(780 nm)LED,用于測(cè)量植物葉片或藻類樣品PAR吸光系數(shù)。

?  監(jiān)測(cè)功能:可實(shí)時(shí)監(jiān)控箱內(nèi)環(huán)境狀態(tài)、機(jī)器狀態(tài)監(jiān)控區(qū)顯示目前程序及環(huán)境狀態(tài)

?  環(huán)境仿真功能:可程序化多組程序功能 (可多至6組),可個(gè)別設(shè)定執(zhí)行天數(shù),可設(shè)備單日環(huán)境參數(shù)(間隔可至分鐘),程序化環(huán)境參數(shù)

?  項(xiàng)目管理功能:預(yù)約存多次測(cè)量的植物影像及參數(shù),延時(shí)(縮時(shí))攝影功能

?  數(shù)據(jù)分析功能:可導(dǎo)出照片文件,可導(dǎo)出葉綠素?zé)晒鈪?shù)Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等


系統(tǒng)設(shè)計(jì)

?  可單獨(dú)作為葉綠素?zé)晒鈨x使用,方便帶到野外田間測(cè)量和成像;也可配置箱體式結(jié)構(gòu),容量300L,能同時(shí)滿足植物培養(yǎng)和葉綠素?zé)晒獬上竦墓δ堋O潴w內(nèi)設(shè)有栽培區(qū),可分2層、3層、4層等,調(diào)節(jié)高度進(jìn)行培養(yǎng)和測(cè)量。箱體內(nèi)有特殊消光圖層,避免反光造成的測(cè)量干擾。

?  系統(tǒng)配置觸摸式高清顯示屏(24寸),方便操作,支持1670萬(wàn)色彩和FHD高清畫(huà)質(zhì)。

?  系統(tǒng)處理器:I7處理器,16G緩存,512固態(tài)硬盤SSD,滿足快速數(shù)據(jù)處理和存儲(chǔ)要求。

?  供電:室內(nèi)使用220V,50Hz交流電,熒光儀單獨(dú)使用時(shí),內(nèi)置可充電鋰電池供電,滿足4小時(shí)的野外測(cè)量


箱體環(huán)境控制(光照、溫度等)

?  溫度環(huán)控條件:15-40°C ±5°C(關(guān)燈)

?  光照系統(tǒng):采用四合一LED光盤,白光5000k ±500k、紅光660nm±10nm、藍(lán)450nm±10nm、紅外光730nm±10nm,光盤面積29×40cm,四種光源可獨(dú)立自主控制,光質(zhì)強(qiáng)度可達(dá)PPFD ≧700μmols-1m-2@15cm,光照時(shí)間控制可程序化設(shè)定,設(shè)定時(shí)間可至1min,可仿真日出至日落光照變化。

?  控制系統(tǒng):PID微電腦控制,可設(shè)定上晝夜循環(huán)程序

?  可選配二氧化碳和濕度控制單元


成像技術(shù)參數(shù)

Maxi探頭

?  熒光測(cè)量光源: 44個(gè)藍(lán)色LED,450 nm,測(cè)量光強(qiáng)度0.5 μmol m-2 s-1PAR,最大光化光強(qiáng)度1900 μmol m-2 s-1PAR,飽和脈沖強(qiáng)度4000 μmol m-2 s-1PAR

?  吸光系數(shù)測(cè)量光源:16個(gè)紅光(660 nm)和16個(gè)近紅外(780 nm)LED,用于測(cè)量樣品PAR吸光系數(shù)。

?  成像面積:工作距離18.5cm,成像面積 10×13 cm;工作距離22.5cm,成像面積11×15cm。

?  光強(qiáng)異質(zhì)性:測(cè)量區(qū)域光強(qiáng)異質(zhì)性小于±7%。

?  測(cè)量參數(shù):Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等。


應(yīng)用領(lǐng)域

?  光合作用研究:可以在完全相同的條件下同時(shí)對(duì)大量樣品進(jìn)行成像

?  植物病理學(xué):病斑部位(包括肉眼不可見(jiàn)時(shí))成像以及病斑擴(kuò)散的時(shí)空動(dòng)力學(xué)

?  植物脅迫生 理學(xué):肉眼不可見(jiàn)脅迫損傷的早期檢測(cè)

?  遺傳育種:出苗后大規(guī)??焖俸Y選高光合/抗旱/抗熱/抗凍/抗病等植株

?  突變株篩選:快速篩選模式植物的光合突變株、抗逆突變株、產(chǎn)氫微藻突變株等

?  微藻毒理學(xué):不同毒物濃度多個(gè)重復(fù)的樣品一次測(cè)完,軟件自動(dòng)計(jì)算抑制比率

?  分子生物學(xué):宏觀水平上檢測(cè)樣品的綠色熒光蛋白(GFP)熒光

?  其它多種擴(kuò)展研究


成像參數(shù)

 Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), ETR, Abs, NIR和Red等

 

突變株的快速篩選

MAXI-IMAGING-PAM特別適合對(duì)幼苗、愈傷組織、微藻等進(jìn)行突變株的快速篩選,適合于與光合突變株、抗逆(抗旱、抗鹽、抗病等)突變株、產(chǎn)油/氫突變株等的快速篩選。

 

國(guó)外利用MAXI-IMAGING-PAM篩選突變株的典型客戶如拜耳、BASF、孟山都、先正達(dá)等大型跨國(guó)農(nóng)業(yè)巨頭,以及各大農(nóng)業(yè)育種、植物分子生物學(xué)等科研單位,例如澳大利亞植物功能基因組中心(阿德雷德大學(xué))、德國(guó)尤里希表型植物表型研究中心(Julich Plant Phenotyping Centre)等等。

 

國(guó)內(nèi)約一半的MAXI-IMAGING-PAM客戶在進(jìn)行突變株快速篩選工作,主要分布于中國(guó)科學(xué)院、中國(guó)農(nóng)科院和各大高校。


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突變株篩選實(shí)例一:國(guó)內(nèi)某客戶篩選的擬南芥突變株

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突變株篩選實(shí)例二:產(chǎn)油突變株的篩選。Ajjawi et al, 2010, Plant Physiol., 152: 529-540.

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突變株篩選實(shí)例三:光合突變株的篩選。Armbruster et al., 2010, Plant Cell, 22: 3439-3460.


調(diào)制葉綠素?zé)晒獬上駥?shí)例

葉片成像異質(zhì)性

1)葡萄葉片

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2)荷花葉片

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水果的成像

1)草莓的成像

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2)獼猴桃的成像

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突變株篩選

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植物病理研究

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熒光成像與CO2氣體交換的同步測(cè)量

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(GFS-3000/IM-MAXI):MAXI-探頭與GFS-3000聯(lián)用,在10 cm x 13 cm的面積上同步測(cè)量氣體交換與熒光成像。


產(chǎn)地:德國(guó)WALZ

 

代表文獻(xiàn)

數(shù)據(jù)來(lái)源:光合作用文獻(xiàn)Endnote數(shù)據(jù)庫(kù)

原始數(shù)據(jù)來(lái)源:Google Scholar

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